Effects of Biotronic® Top3, a feed additive containing organic acids, cinnamaldehyde and a permeabilizing complex on growth, digestive enzyme activities, immunity, antioxidant system and gene expression of barramundi (Lates calcarifer)

The Biotronic® Top3 (OCAP: organic acids, cinnamaldehyde, and permeabilising complex) is used for improving microbial community in monogastric animals. This research was aimed to test the five dosages of OCAP (0%: control, 0.05%: OCAP0.05, 0.10%: OCAP0.1, 0.15%: OCAP0.15%, 0.20%: OCAP0.2) barramundi (Lates calcarifer) (8.3 ± 0.3 g) eight weeks. results showed a linear relation between level growth rate, feed conversion ratio, protease, lipase, lysozyme serum mucus, alternative complement activity (ACH50) serum, catalase, glutathione peroxidase, malondialdehyde levels (P < 0.05). Accordingly, OCAP0.2 treatment had best performance abovementioned parameters. While there no significant difference expression heat shock proteins (HSP70, HSP90) tumour necrosis factor (TNF-a) among groups, interleukins (IL-1β, IL-8) were significantly lower OCAP0.15 group than others. IGF-1 followed rate trend. Generally, feeding fish with diet improved digestive enzymes activities, immune response, antioxidant system, gene expressions barramundi. As observed, supplementing more reach plateau recommended.